Hi everyone,
Due to poor water solubility of Nebivolol, I wonder if someone knows the possibility of dissolving it in Tween 20 for cell culture. Does it work or may it be toxic for PBMCs?
Best regards,
Mehri
Hello Mehri Hajiaghayi
Nebivolol is a lipophilic substance, and you can dissolve it in 100% DMSO. You can make a stock solution (say 1mM) in DMSO and store it in -20˚C. The working concentration of Nebivolol can be made in cell growth medium. The final concentration of DMSO in cell culture that is not toxic to the cells should be 0.1% -0.5%.
Good Luck.
Malcolm Nobre
Many thanks for your answer.
We already used DMSO as a solvent for nebivolol but the results of our cell culture show that DMSO may have a direct role in T cell response by itself. So, we are looking for finding other types of solvents.
Have you tried DMF and methanol. You need to take care of the cytotoxic effects on the cell.
You're right, we can try these two solvents too.
I've read that tween 20 would be toxic in less that 0.1% .
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