Hello fellow researchers, have you ever performed RNAseq from flow sorted fixed permeabilized cells after intracellular staining.
Hello Manojkumar Gunasekaran
Did you make any progress?
Thanks,
Hope
Hi all, I am working on mechanistic study of organometallic complexes using Gaussian 09 program package. In most of the recent research paper they reported that LAC3Vp basis set is good for...
03 September 2023 8,699 3 View
Please explain the consequences of Charge resistance and warburg resistance influence catalytic activity ? Mainly in water splitting reaction .
15 March 2023 610 0 View
Compare to C-D bond O-D bond is weaker. According to catalytic cycle (attached below) O-D bond cleavage is not occur? Could please explain the reason? Reaction condition: Pd(OAc)2/ Catacxium...
23 January 2023 9,718 3 View
Bare nickel foam giving the good activity about 280 mV at 10mA current density . How people mentioning lower activity of Bare nickel foam ? After the acid Wash also .
02 January 2023 8,077 0 View
I am new to KiSThelP software which is used to calculate the rate constant of a reaction. I have the gaussian output file for the reactant, transition state and product. Can anyone help me to...
26 February 2021 8,546 1 View
This is for understanding from engineering point of view, if i have only current measurement at site, can I make a safe design by making safe estimates of temp, salinity or turbidity > will it...
25 November 2018 5,339 0 View
Bio molecules are less stable compared to Pharma drugs. Since proteins are notorious for stability many impurities gets generated on the stability. If impurities are not available, we need to...
22 March 2018 6,649 3 View
How one can find out the list of predatory journals/publishers (like Beall's list)?
11 March 2018 6,129 3 View
If such any research articles are available please share it……………..
09 February 2018 9,626 4 View
I know about collection of tissue samples from normal as well as disease tissues. Here I wanted to know how the same is collected in terms of blood? Also, how detection of somatic mutation...
10 July 2017 1,217 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View