Hello Friends, How much % of impurity need to be generated in forced degaradation (FDS) of Biomolecules?

Bio molecules are less stable compared to Pharma drugs. Since proteins are notorious for stability many impurities gets generated on the stability. If impurities are not available, we need to...

22 March 2018 6,414 3 View

Is there any updated list of predatory journals/publishers available on the Web (like Jeffry Beall's)?

How one can find out the list of predatory journals/publishers (like Beall's list)?

11 March 2018 5,954 3 View

Is there a possibility to increase the benefit cost ratio (BC ratio) of the horticultural crops using fuzzy Logic?

09 February 2018 9,380 4 View

Availability of Somatic Mutation Gene Panel in Breast Cancer?

Is there any somatic mutation gene panel for detecting breast cancer? I already know there are Germline mutation gene panel for Breast Cancer

09 July 2017 5,361 1 View

How to separate G and D' peaks of highly defective Graphene or vertical graphene nanowalls in Raman spectroscopy.

21 June 2017 3,672 2 View

How to purify all the nucleic acid (DNA and RNA) associated protein from bacteria?

22 September 2016 3,836 2 View

Is cloning a 9kb 3'UTR for luciferase assay necessary or cloning the regions of 3'UTR-miRNA interaction is sufficient?

08 February 2016 3,164 3 View

What is the optimum irreproducible retention time of the peak in HPLC?

I am doing reverse phase HPLC using C18. Keeping the flow rate and composition of solvent constant, I should get my target peak at 14 min, but I am getting the peak sometime at 14 minutes sometime...

27 June 2014 4,361 4 View

Is a discrete time system stable if and only if all the roots are located inside the unit circle z<<1?

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21 February 2014 1,651 9 View

Does anyone have experience with using the ligation mix is directly to transfect cells?

Basically, transformation of ligation mix into E.coli is meant to increase the amount of DNA to be used for transfection. But is it possible and if possible what are the consequences of using the...

27 June 2013 707 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View