What's wrong with my sample stained with MAP2 antibody?

I've studied and fabricated a nanostructured substrate for the enhancement of neuronal differentiation. I usually confirmed the levels of differentiation using immunocytochemistry, but I recently...

20 September 2020 5,725 3 View

How can I analyze height of nano-pattern sample through scanning electron microscopy (SEM)?

Hello, I'm fabricating nano-pattern through LIL(laser interference lithography). I want to check the height of pattern sample through SEM, since our lab has it. I've tried to check the height of...

01 July 2019 2,733 3 View

Is it possible to prove that cations are complexed in the solution by using UV-visible spectroscopy?

Hi, i just bring a one question. As i said, Is it possible to prove that the cations are complexed in the solution by using UV-visible spectroscopy? For example, assuming that there are two...

27 November 2018 4,895 3 View

Can the electrophoresis of a peptide whose net charge is negative charge?

The proteins I have studied so far have been confirmed by electrophoresis without any problems and all have positive positive charges. And also, markers sold on the market also consist of proteins...

06 September 2018 6,198 2 View

How can i draw ion concentration versus pH graph?

Hello, i'm here to ask about this, drawing the graph. I have searched lots of articles which show the graphs similar with attached image. However those articles does not have any information about...

22 August 2018 9,045 4 View

Can I determine the concentration of silver nanoparticles using UV-visible spectrometry?

We were given a few samples of silver nanoparticles (AgNPs) samples (of different colours, green and yellow solutions) for the investigation of the antifungal efficacy of AgNPs. The AgNPs were...

21 December 2017 1,389 2 View

How can I increase structure properties to carbon material?

i want to make the hollow shaped carbon materials. when i analysis it, but it collapse the wall. so i think coating like polymer at the the carbon morphology outside. but i'm not have imformation...

27 June 2016 6,507 3 View

How to make the sampling graphene for TEM?

13 June 2016 2,990 3 View

Adding HEPES to medium

I'm using DMEM supplemented with 10% HI FBS and antibiotics for J774A1 cell line. There is no HEPES in DMEM used in our lab. After seeding and serum starvation during 12 h, J774A.1 cells were...

12 June 2012 6,083 7 View

The shape of J774A.1 cell line

Hello, This picture is J774A.1 cell line. I wonder whether the cells on the right side of picture, and middle (upper) of picture are stressed. They are cultured in DMEM with 10% FBS and 1%...

24 May 2012 7,801 4 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View