Yes. Normal plastic dishes can do, but if intention is to differentiate into myotubes can also use gold-plated wells. A lot of factors influence differentiation.

Our recent work in 6-well plates:

Article Interleukin-6 Induces Myogenic Differentiation via JAK2-STAT...

@Joo Yeon Lee,

C2C12 can be easily grown on 96 well plate. Since it grows faster, 5000 cells will give you optimum seeding density.

If you planning for myotube differentiation, than I recommend to use 12 well plates as all the cells doesn't differentiate.

Jayant Dewangan

So, Is it impossible myotube differentiation in 96 well plate?

@Joo Yeon

It will form but microscopic fields will be very less.

Another thing for differentiation, you need to start treatment with 90-100% confluence.