Why do we only study the DC gain in VLC channel modeling?

In all the papers and books that I read, only the DC channel gain of the VLC system is studied without any further justification. How can we know for sure that the channel doesn't affect the...

13 February 2020 6,718 3 View

Cell line cross contamination, are these HEK293 cells (picture)?

Dear all, I am culturing Sprague Dawley Rat bonemarrow MSCs, we ordered them from cyagen, 2nd passage. We had contamination problems with another cell, possibly HEK293 cells, which we discovered...

27 February 2019 7,632 3 View

What is the best algorithm to identify miRNA sites in the 3'UTR of a mRNA?

I tried TargetScan, microRNA.org, PicTar and Targetspy and I got different results. Also, when you present the results, do you mention only the conserved miRNAs or the poorly conserved as well?...

20 March 2014 3,211 3 View

Can anyone help with murine mesothelioma immunolabeling?

I would like to characterize murine peritoneal mesothelioma using immunolabeling on paraffin-embedded tumor. Can someone indicate me references of primary antibodies that worked, such as antibody...

01 December 2013 4,011 1 View

How do I determine the molecular mass of smectite?

I have analysed clay particlesn with XRD and a peak associated with smectite was seen. I want to work out how much smectite could be in the clay by using XRF data I also obtained.

13 October 2013 2,416 2 View

Can anyone help me with my dot blot analysis?

Performing dot blot with cellular extract and western blot anti-GAPDH, we have obtained irregular labeling of our dots with white labeling in the center. How to explain this labeling? 2.5 ul of...

12 November 2012 7,234 12 View

IL-1 beta antibodies used in western blot

I would like to detect secreted IL-1 beta from human cell supernatant using Western blotting. Has someone tested antibodies directed against IL-1 beta working in WB? Can you recommend references...

24 April 2012 1,992 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View