Hi everyone,

I am looking for some information regarding spinal cord sectioning to examine changes within the inhibitory interneuron population.  I am used to sectioning brain tissue, but this will be my first time working with mouse spinal cord.  Animals were perfused with 4% PFA and samples were left in PFA overnight.  They are currently stored in PBS + 0.06% sodium azide.  I usually put brain tissue in 30% sucrose 3-5 days before slicing...would I do the same for the spinal cord? Would the slicing protocol be similar for the spinal cord vs. brain tissue?

Any assistance is greatly appreciated!  Thanks!