Hi everyone,
I am looking for some information regarding spinal cord sectioning to examine changes within the inhibitory interneuron population. I am used to sectioning brain tissue, but this will be my first time working with mouse spinal cord. Animals were perfused with 4% PFA and samples were left in PFA overnight. They are currently stored in PBS + 0.06% sodium azide. I usually put brain tissue in 30% sucrose 3-5 days before slicing...would I do the same for the spinal cord? Would the slicing protocol be similar for the spinal cord vs. brain tissue?
Any assistance is greatly appreciated! Thanks!
Yes, you may use the same protocol. To my knowledge, slicing protocol for both spinal cord and brain tissues should be the same. 30% sucrose will prevent shredding of your samples while sectioning.
Guru.
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