Hello everyone,
I'm using calorimetric LDH assay to determine the % of lysed HCT116 cultured in RPMI media supplemented with 10% FBS for 24h. I used the same growth media as background control. Then I found out that the LDH absorbance in the background control is higher than in HCT. May I know if this is possible?
Was your culture medium background pH-controlled? Meaning, was it from the fridge or was it from the same plate that the cells were on in the incubator?
The pH indicator may be the cause, and if the pH isn't the same or at least close it could have a significant effect assuming you are doing something like a 1:2 or 1:3 ratio of medium to LDH assay reagent.
Dear Jordan,
Yes, it was from the same plate-meaning that it was incubated at the same condition as in HCT116
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