I don't understand from your question why this is a 2x2x2 factorial or what all the variables are. I recommend this book: https://www.amazon.com/Analysis-Experiments-Enhanced-Abridged-Companion/dp/1119593409/ref=sr_1_1?keywords=Douglas+montgomery+design+of+experiments&qid=1574883206&s=books&sr=1-1 and the statistical consultant at your University. Best wishes, D. Booth