Hello,
Many researchers reported that to extract polyohenols you need first to remove lipids. I don't know why? Knowing that removing lipids can leads to a loss of polyphenols even if the solvent is apolar.
You can try this protocol to extract polyphenols.
Article Antioxidant activities, polyphenol, flavonoid, and amino aci...
Hi Oumayma,
Generally polyphenols are polar than lipids and hydrocarbons, so using a non polar solvent as hexane will help to remove lipids and hydrocarbons. You will then use dichloromethane to extract the polyphenols.
You can try this protocol to extract polyphenols.
Extraction of Polyphenols from Olive Pomace In brief, one hundred grams of the dried defatted olive pomace (ddp) were extracted with aqueous methanol (80%) using solvent/pomace ratios (v/w) of 20:1 for 7 min (Technique 1) and 40:1 for 30 min (Technique 2), at room temperature. UAE was conducted using Fisher-Sonic, Dismembrator Model 300, N.Y., USA at 50% of the maximum output power (300 W). The resulting slurry was centrifuged at 3000g. After filtration, the solventwas removed under vacuum at 40°C using a rotary evaporator (Eyela, Japan). Subsequently, the concentrate was kept at −18°C for further analysis.
Hello Oumayma
Lipid compounds can generate a barrier in the extraction of polyphenolic compounds, due to their apolar nature. They would not allow the polar interactions that are required between polyphenols and the solvent used as extract.
Hello,
it is generally useful to remove the lipids with e.g. hexane in order to come to a good separaton of polyphenolic compounds. You will not lose any polyphenols when you check e.g. the hexane phase for secondary compounds (e.g, for phenolics).
Kind regards, Klaus Peter
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Extraction