I am extracting DNA and RNA from FFPE tissue, matched colorectal metastasis, primary tumour and matched normal tissue. (RNA is complete, with the DNA I am going on to do exome sequencing and methylation arrays.)
I have used the Covaris truXTRAC® FFPE DNA and RNA Kits, some samples were low concentration (I am aiming for 300ng/50ul) and so I repeated them, and then again for those that were still low with the Covaris truXTRAC® FFPE DNA kit.
I have noticed there is a greater proportion of normal tissue which is of low contraction (10 samples vs. 4) and wondered if this is because it is mainly adipose tissue e.g. omentum?
I wonder if anyone has any experience / can recommend a different extraction method to maximise the yield from this tissue? (FFPE, adipose tissue)
Many thanks,
Sally
Dear Sally, we used RNeasy FFPE kit (qiagen) for purification of total RNA from formalin-fixed, paraffin-embedded tissue sections. It works well.
Thankyou for your answer, apologies I should have been clearer I am looking to repeat the DNA extractions only
Have you tried with ¨Infinium FFPE DNA Restoration Solution¨ by Illumina?
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