How to find old and well-cited papers?

How to find papers via ReseachGate such that 1. the paper is at least 5 years old and 2. has a high number of citations (a) in general, (b) in the last 2 years?

30 April 2020 3,045 12 View

Please tell me how can I add PowerVmAllocationPolicyMigrationMedianAbsoluteDeviation as VM allocation policy?

Datacenter datacenter = null; try { datacenter = new Datacenter(name, characteristics, new PowerVmAllocationPolicyMigrationMedianAbsoluteDeviation(), storageList, 0); } catch (Exception e) {...

13 December 2016 7,072 1 View

Image analysis to count and measure the diameters and fluorescence intensity of immunofluorescent neuronal somata?

I have fluorescent images with labeled neurons and I need to count, analyze and sort them by size and fluorescence intensity. I have plenty of these images so I don't want to do this manually with...

15 November 2013 1,600 6 View

Difficulties working with Calcium Crimson?

We've been trying to run calcium imaging experiments with the red Calcium Crimson and somehow it does not load our neurons well. We are quite successful with Oregon Green BAPTA and Fluo4. These...

15 November 2013 257 3 View

Complain against journals

Where can an author complain against a journal if the journal behaves in an unethical way according to the opinion of the author?

01 January 1970 6,040 9 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View