We are expressing a transporter in Pichia X-33 but the western showed to much proteolysis. Suggestions?
Is is a secreted protein?
No, is a Golgi membrane transporter
I am working with secreted proteins and it seems like longer expression times lead to an increase in protein degradation. Maybe you could try an expression time course and see if shorter expression times give you a cleaner protein?
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Cuáles fueron las tendencias en investigaciones en arquitectura, urbanismo y patrimonio edificado en decadas del 2000 al 2020? Porque requiero conocer tesis de posgrado nivel maestría...
24 July 2024 5,494 1 View
I synthesized pure ZnO with a hydrothermal microwave method and decorated them with CuO with the incipient wetness impregnation method. The Zn2p edges of pure ZnO are at 1044.7 and 1021.6 eV...
18 June 2024 9,405 2 View
One of the most intriguing frontiers in contemporary research is the application of Generative Artificial Intelligence (GenAI) in qualitative research. This technological advance offers tools that...
13 June 2024 781 2 View
I'm currently doing an experiment where I'm following the expression of a reporter gene (GFP) along growth using S. cerevisiae. The cultivations last around 48 hours. I observed that the results...
10 June 2024 5,941 3 View
Does anyone have the full text of this article? KELIKIAN H, RIASHI E, GLEASON J. Restoration of quadriceps function in neglected tear of the patellar tendon. Surg Gynecol Obstet. 1957...
08 June 2024 7,054 0 View
It is difficult, or even impossible, to find real performance metrics (transactions cost, time to transaction finality, transactions per second, etc.) on the different blockchains. In their...
13 May 2024 10,182 3 View
I'm trying to perform the sulphu-phospho-vanillin assay for total lipid content in microalgae. I'm having trouble with the methodology which I'm not able to understand well when it comes to the...
25 April 2024 6,932 0 View
I have searched the scientific literature, and I cannot find information about it, I have only found information about metallic nanoparticles, with gold and other metals. If anyone has information...
17 April 2024 7,604 2 View
I am currently engaged in a research project aimed at evaluating the expression and secretion of osteogenic differentiation markers in mesenchymal cells. My intention is to investigate...
04 April 2024 6,251 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,294 3 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View