How should I dispose of silver staining waste (SDS-PAGE)?

I know I cannot pour silver staining waste down the sink, but how should I dispose of it? Thank you in advance!

18 November 2020 1,745 3 View

Do you know a not very expensive journal of Metagenomics?

Hi you all, I'm looking for a not very expensive journal to submit our work related to microbial diversity in a beverage drink using an amplicon perspective. Any suggestion is welcome because I...

02 May 2020 2,165 4 View

Can you help me with a lab protocol (RT-PCR, capillary electrophoresis, etc) to detect the fusion gene FIP1L1-PDGFRa in hypereosinophilia disorders?

I need a molecular biology protocol to detect this fusion gene FIP1L1-PDGFRa in hypereosinophilia disorders, because the protocol that I have is not working, and I see several inespecifical bands...

13 February 2020 1,304 4 View

Did anyone of you ever resuspended compounds such as beta sitosterol or campesterol in culture medium?

I noticed that these compunds are not soluble in DMSO, and DMSO damages hepatocarcinoma cells a lot. Thank you!

04 February 2020 6,575 1 View

How many mice do I need to do qPCR of T cells sorted from B16 TILs?

03 February 2020 9,215 2 View

Why is the TMB substrate turning blue without being in contact with the sample and in the dark?

Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...

03 January 2020 1,294 7 View

How to prevent 68Ga-colloid formation?

Hi, I am currently trying to optimize the radiolabeling of a peptide (w/ NOTA) but I am ending up with 30% 68Ga-colloid in the reaction. I am using HEPES as the buffer and setting the reaction pH...

06 October 2019 1,181 2 View

How can i know if my cell type expresses a certain type of enzyme?

In an informatic database, like typing the name of the enzyme and then the information of the enzymes expressed in that particular cell type.

16 May 2019 2,893 3 View

What antibody is used for the E. histolytica amebiasis antibody test(blood)?

I am trying to see if I can test the serum of infected mice with this antibody

15 January 2019 8,257 6 View

Is my panel data set appropriate for GMM?

I have a panel data set with N=22, T=15 Although N>T, is my N=22 appropriate for GMM estimations? Thanks

10 November 2018 806 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View