Hi Sneha, if I remember correctly exosomes are cell membrane blebs that are pelleted from serum using ultracentrifugation.

This is not so different than isolating lipoprotein particles. Centrifuge, resuspend the pellet in PBS and recentrifuge. Just be aware that you are pelleting lipoprotein particles along with the exosomes.

Hey Sneha I would reccomend using the The Invitrogen Exosome

Isolation kit (for serum). Our lab has compared this kit with ultracentrifugation and we have shown that the kit gives us more pure exosomes and higher yield compared to ultracentrifugation. This is the link to our publication which we have shown this: http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path%5B%5D=23455.

This is the link for the the The Invitrogen Exosome Isolation kit for serum: https://www.thermofisher.com/order/catalog/product/4478360

Best of Luck,

Kevinn

Hi Sneha,

I have worked with exosomes isolated as described in the following article:

Pérez-González R., Gauthier S.A., Kumar A., Saito M., Saito M., Levy E. (2017) A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space. In: Hill A. (eds) Exosomes and Microvesicles. Methods in Molecular Biology, vol 1545. Humana Press, New York, NY

I highly recommend this procedure.

Good luck,

Hediye.