Hello everyone.
I would like to assess insulin signaling in adipose tissue of mice. Do I have to starve animals prior sacrifice or not? Or may I put tissue in proper buffer/medium, right after explant, even if I did not starve animals? Perhaps I can leave adipose tissue without nutrients for a period of time to mimic starvation and then add insulin and after 15-30 minutes assess its action, does it make sense?
I think it is best if you would fast the animals overnight. This will give you a more standardized way
I think that insulin and leptin are the hormones of fed state therefor atleast 14 hours of fasting of your animal may wash out endogenous insulin, however stress of sacrifying may interfer here because you may have another local surge of insulin after stress induced hyperglycemia. Therefore your second option and translated starvation may be better.
Dear Colleague.
I am working on very local field of exp.diabetology as investigation of mechanisms of diabetes caused by some chemicals that is why I am not belong to specialist, unfortunately, corraspond to your scientific interests. Sorry!
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