Salvatore Saieva

3 Questions 3 Answers 0 Followers

Questions related from Salvatore Saieva

How to homogenize corneal tissue?

Dear All, I would like to homogenize cornea tissues from human patients. I need the homogenate as it is, because I want to inject it in mice, therefore I am not using any detergent in the buffer...

27 June 2025 6,107 0 View

Astrocytes: morphometric analysis of extracellular processes (length, number...). How can I do it?

Dear All, I performed an immunohistochemistry in mouse brain tissue looking for astrocytes (stained for GFAP). Now, I would like to evaluate any morphologic change between the different groups I...

01 October 2019 9,019 3 View

Ex vivo insulin response assessment on mice-derived tissue samples?

Hello everyone. I would like to assess insulin signaling in adipose tissue of mice. Do I have to starve animals prior sacrifice or not? Or may I put tissue in proper buffer/medium, right after...

13 March 2017 3,315 4 View