Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

How should private health-care systems be improved to guarantee everyone's right to health?

COVID-19 pandemic has shown big social inequalities in countries with private health-care systems. Governments must pay greater attention to the right to health, that every human being must have,...

31 May 2020 7,357 23 View

I'm trying to set up a TR-FRET experiment in my lab but I'm having some troubles. Could you give some tips?

I'm working with an his-protein and I'm trying to obtain an IC50 between the protein and the biotinylated substrate and a known competitor. But the experiment is not working. I will add the...

20 April 2020 4,485 3 View

Problems with Google Scholar. Do you know how to fix it?

Dear All I am having some problems with google scholar. Some citations to my papers simply disappeared. I know that the number can be reduced when two different versions of a paper are...

01 July 2018 873 60 View

Is organic chemistry predictable?

It seems like organic chemistry consists of learning a bunch of reactions, but is there any qualitative set of rules for predicting the way a set of organic reagents will react? I know that...

30 May 2017 3,980 3 View

Are there any recognized / respected certificates for R programming or data analysis ?

Thinking about online and MOOC-type certificates for R programming and data analysis, are there any that are recognized and respected by potential graduate schools and employers? I guess if people...

11 March 2017 8,044 3 View

Hi all, what is the best method to determine the onset of bird migration and the end (day arrival), using GPS points?

There is a clear evidence in my data, but I would like to have also a statistical evidence

17 September 2016 7,140 4 View

Has anybody used Cas9 knockin mouse? If so do you know if every single cell express Cas9 constitutively?

Has anybody used Cas9 knockin mouse? If so do you know if every single cell express Cas9 constitutively?

29 September 2015 5,799 3 View

Is there a Cre line specific for the immune system?

22 June 2015 7,815 2 View

Is it possible to conduct AFM electrical measurements (such as EFM and KFM) in liquids?

I was wondering is any of the above techniques can be used for measurements in liquids. Thank you!

19 January 2015 3,178 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View