Hello everyone, Does anyone know of a laboratory or researcher in Europe who performs quantitative PCR for Cryptosporidium sp?

More Salvatore Scarso's questions See All

Are there clinical trials concerning neurogenesis and the use of stem cells for patients with subarachnoid haemorrhage, of non-traumatic origin?

Dear Community Members, I'm reaching out to you, especially to those specializing in the medical field, for information regarding clinical trials on patients who have suffered from the rupture of...

04 June 2024 1,465 0 View

Have there been any studies of suicide or suicidal behavior among the personnel of county sheriff's offices in the United States?

There have been several suicides in the US in late 2023 and in the first several weeks of 2024 involving deputy sheriffs and, in one case, a county sheriff. There is a large a growing literature...

17 February 2024 7,147 1 View

Is "mastering a foreign language" a soft-pure or soft-applied discipline?

Within the categorization of soft-pure and soft-applied disciplines, I never see a clear reference to “foreign language learning”. Perhaps this is due to the distinction between the five (or even...

20 January 2024 8,293 2 View

Nanozymes were the basic chemical constituent that kicked off the origin of life??

⚪️⚪️ENG-TEXT⚪️⚪️ The question (hypothesis) proposed by this Open RG Question is the following: Nanozymes were the basic chemical constituent that kicked off the origin of...

01 November 2023 7,760 2 View

How to prepare synthetic livestock or dairy wastewater?

Synthetic wastawater: ammonium nitrogen, COD and BOD

07 October 2023 6,475 2 View

How do you justify the preference for one Bibliographic Database above others?

As is well known, Scopus and WoS, unlike many other bibliographic databases, offer very precise filtering functions. It may happen that a peer-reviewer asks to broaden the search to prove that...

11 September 2023 2,284 3 View

What were the personality traits most admired in ancient Rome around the first century of the common era?

I am working on a comparative analysis of "most admired personality traits" in N.T, Greek culture and Roman world

17 August 2023 10,008 0 View

Is there a standard definition of "peer reviewer"?

There is a great deal of information available about the peer review process but little (that I could find) about exactly what the qualifications are to be a peer reviewer, other than that...

20 June 2023 2,688 5 View

How do I replace a paper with a more updated version of the same ?

After uploading the main text of a paper, I received from the Journal a corrected version and I want to replace the old text

27 April 2023 8,534 1 View

Any evidence or thoughts as to whether or not owning owning a semi-automatic civilian version of an "assault rifle" raises the risk of suicide?

Access to firearms is a well documented risk factor for suicide. Regardless of why someone might have one or more of AR-15 or similar weapon, there's no disputing their inherent lethality....

08 June 2022 7,117 7 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Can i apply a public questionnaire but analyse it with a different data analysis method?

First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...

27 July 2024 3,457 3 View

How to merge two or more linkage maps?

I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.

25 July 2024 6,505 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View