Hello I tried checking Protbase Expasy etc but I am a noob in proteins :) So I am looking for EGF protein NOT EGFR. As far as I see its a single pass type 1 membrane protein.
Since EGF binds to EGFR and continues downstream reactions would this mean this EGF gets excreated from the cell? Or can EGF and EGFR stay in their place and still get connected? It must sound silly but would a single pass type 1 membrane be introcelluar or extracelluar when you know it has a receptor somewhere that it needs to bind?
Thank you
Mature EGF is an extracellular protein, released by proteolytic cleavage from a membrane-bound precursor - the original transcript contains an N-termina signal peptide (1-22), a transmembrane helix (1033 – 1053) and a cytoplasmic domain (1054 – 1207).
Mature EGF consists of residues 971 – 1023 of the precursor, released by proteolysis from the membrane-bound precursor. The precursor consists of several EGF-like repeats, interspersed with LDL-receptor classB repeats. Considering how much of the surface of EGF is buried in the complex between EGF and EGFR, and how close to the membrane (30 residues) the EGF is located in the precursor, I consider it rather unlikely that EGF in the context of the the EGF precursor can bind to the EGF Receptor.
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