Hello everyone,
I am culturing MDA-MB-231 cell line in Leibovitz's l-15 medium supplemented with 10% FBS and 1% Pen/Strep in an incubator at 37°C without humidifying pan. I observed that some of the culture medium vaporizing during culturing and water droplets on the upper surface of the flask. How can I solve the evaporation from the culture medium without disturbing the cells?
Sorry for the inconvenience.
Thanks in advance,
Mervenur