Mervenur Yavuz

4 Questions 4 Answers 0 Followers

Questions related from Mervenur Yavuz

Selection with Puromycin for generating stable cell line?

Hello everyone, In a project I was working, we generated a construct containing Puromycin resistant gene. I want to transfect my GBM cells and select the cells with stable expression of the...

16 January 2022 5,626 1 View

Evaporation from cell culture medium?

Hello everyone, I am culturing MDA-MB-231 cell line in Leibovitz's l-15 medium supplemented with 10% FBS and 1% Pen/Strep in an incubator at 37°C without humidifying pan. I observed that some of...

20 December 2021 3,265 6 View

How to generate Autodock Grid Box?

Hello everyone, I am trying to do molecular docking using AutoDock Software. One of the struggling I am trying to deal with is generating Grid Box. How can I generate Grid Box without knowing...

09 November 2021 807 7 View

My SVG p12 cells are dying when I passage them into a T75, I couldn't find a solution for that issue. Could anybody please give any advice for it?

I am culturing SVG p12 cells in %10 FBS, %1 Pen/Strep DMEM medium. But whenever I passage them into a much larger flasks they are began to de-attached and started to die. I couldn't find any...

17 January 2021 4,747 4 View