You cannot accurately measure absorbance in the presence of bubbles or suspended solids, including colloids

The bubbles will have a refraction on the light, which will also cause the light intensity to decrease after passing through the solution, and the absorbance to increase, resulting in a larger concentration of the measurement.

Dear Shubang Vyas, these bubbles ususally cause significant light scattering that shifts the entrire spectrum upward but it usually does not change the shape of the spectrum. I have no idea on how much bubble is produced in your system, but If the gas bubbles are not produced so fast and they only attach to the walls of the cuvette, you can degass the solution by dipping the cuvette in an ultrasonic bath for 1 or 2 seconds and immediately record the spectrum thereafter. You might also use some scatter correction techniques but since the production of the gas bubbles is not repeatable, I think this approach is rather impractical for you. A similar topic has been discussed before in Researchgate that you can find its link down below.

https://www.researchgate.net/post/What_is_the_effect_of_the_light_scattering_on_the_absorbance

Best,

Dear Shubang,

thank you for sharing this interesting technical problem on RG. I fully agree with the previous answers. I suggest that you just wait until the reaction is over and the bubble formation has ceased. Slight heating will speed up the reaction and cause the bubbing to end. Are you followng a literature procedure?

Good luck with your experiments!

Thank you experts for taking interest in my question. The answers are very useful for my experimental work.

Thanks to all.