Imported CAD geometry not properly simulating in COMSOL?

I am using COMSOL to conduct heat transfer studies for a solar module. I have drawn the separate geometries in AutoCAD and imported them into COMSOL. However, after simulation, the edges of the...

10 June 2020 2,251 5 View

Is it possible to calculate heat production per layer in COMSOL?

I am simulating a PV module which consists of 7 layers. I want to do Surface-to-Surface Radiation and Heat Transfer in Solid calculations. Is it possible to calculate how much heat each layer...

19 February 2020 521 1 View

How can I make a component transparent in COMSOL?

Is there a way to make a component in COMSOL transparent (so you can see other components behind it), but also give that component some color? For example, a transparent glass layer with a blue hue.

12 December 2019 5,910 1 View

Hello, looking for TrpV1 knockout extracts from rat or human cells anyone have any?

Hello, I am testing some TrpV1 antibodies and would like to use KO extracts. I can find some whole mice but they are a bit steep for just some westerns. Does anyone have some KO extracts from...

09 October 2019 9,798 1 View

Can anyone recommend a good ELISA for COL2A1 (human)?

I have a small amount of money and time left on my project and would like to do an ELISA. I'm struggling to find one with any reviews and none of my colleagues can recommend one.

03 December 2018 2,508 2 View

Why does the mean droplet size of an emulsion changes with increasing the concentration of solid particles?

I am evaluating the effect of nanoparticle on emulsions. It was observed that when the increasing the concentration of the nanoparticles changes the mean droplet size (morphology). What is the...

24 July 2018 5,502 13 View

I have a question about a hierarchical GLMM. Can anyone help with some SPSS Genlinmixed syntax?

I'm working up to fitting a Tweedie GLMM in R (mgcv). At the moment I'm playing in SPSS to sort out the data and some ideas for random effects. The data are from a survey of coastal dolphins on a...

10 February 2016 3,747 1 View

Neural network outputting average values of all outputs - Am I doing anything wrong?

14 January 2016 6,803 8 View

I am looking to compile a citation reference list for RNA-Seq; has anyone started one? Would anyone like to collaborate?

Hello, I would like to compile a citation reference list covering human RNA-Seq needs for researchers. I am wondering if anyone has already compiled a "guide line" list for the sequencing depth...

10 January 2016 1,370 2 View

How do I find degenerate PCR primers?

31 August 2015 3,824 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View