Dale Allen

2 Questions 3 Answers 0 Followers

Questions related from Dale Allen

Do I need to wash away VSV-pseudotype virus after 1hr transduction period to study cellular viral entry?

So I have target cells to transduce gene for a VSV-SARS-CoV2-GFP spike pseudotype and have lower infections/transduction of genes when removing the inoculum, however the infection is more...

27 August 2021 220 0 View

Why hACE2 nonspecific binding in HEK293FT cell line?

Hello, I am optimizing a flow cytometry experiments for hACE2 using an RND systems polyclonal goat antibody (0.25ug/1Mcells same as spec sheet and already tried high and low concentrations, to no...

08 March 2021 4,309 3 View