I am trying to digest adult mouse brain tissue for cell isolation, and I am having a hard time getting past the enzymatic digestion step. Previously I used the Dissociation Kits from Miltenyi Biotec, but I do not have access to that right now. I am now using a combination of DNAse I and Collagenase, along with trypsin to aid the digestion process but I am have little luck. If anyone has any tips I would greatly appreciate it.

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