Why do we only study the DC gain in VLC channel modeling?

In all the papers and books that I read, only the DC channel gain of the VLC system is studied without any further justification. How can we know for sure that the channel doesn't affect the...

13 February 2020 6,718 3 View

Cell line cross contamination, are these HEK293 cells (picture)?

Dear all, I am culturing Sprague Dawley Rat bonemarrow MSCs, we ordered them from cyagen, 2nd passage. We had contamination problems with another cell, possibly HEK293 cells, which we discovered...

27 February 2019 7,632 3 View

What is the best algorithm to identify miRNA sites in the 3'UTR of a mRNA?

I tried TargetScan, microRNA.org, PicTar and Targetspy and I got different results. Also, when you present the results, do you mention only the conserved miRNAs or the poorly conserved as well?...

20 March 2014 3,211 3 View

Can anyone help with murine mesothelioma immunolabeling?

I would like to characterize murine peritoneal mesothelioma using immunolabeling on paraffin-embedded tumor. Can someone indicate me references of primary antibodies that worked, such as antibody...

01 December 2013 4,011 1 View

How do I determine the molecular mass of smectite?

I have analysed clay particlesn with XRD and a peak associated with smectite was seen. I want to work out how much smectite could be in the clay by using XRF data I also obtained.

13 October 2013 2,416 2 View

Can anyone help me with my dot blot analysis?

Performing dot blot with cellular extract and western blot anti-GAPDH, we have obtained irregular labeling of our dots with white labeling in the center. How to explain this labeling? 2.5 ul of...

12 November 2012 7,234 12 View

IL-1 beta antibodies used in western blot

I would like to detect secreted IL-1 beta from human cell supernatant using Western blotting. Has someone tested antibodies directed against IL-1 beta working in WB? Can you recommend references...

24 April 2012 1,992 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View