23 October 2012 12 10K Report

Have anyone tried EMSA on Agarose?

I have started to do EMSA on Agarose and I am confused regarding few things.

Is there any specific agarose for Proteins? What protein concentration should be used? I have tried with 2ug/ul but did not get any band.

What DNA concentration should be used for EMSA on Agarose to check whether DNA is being retreaded or not?

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