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Questions related from Farah Deeba
I am doing EMSA by using DIG labelled kit from Roche. I annealed and labelled the oligos according to kit manual, checked labelling efficiency, it was good. Than I did EMSA with nuclear extract...
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Can we use single stranded probe for EMSA for transcription factor identification?
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Have anyone tried EMSA on Agarose? I have started to do EMSA on Agarose and I am confused regarding few things. Is there any specific agarose for Proteins? What protein concentration should be...
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I am doing EMSA to check DNA-Protein interaction. Whenever I run the gel, proteins always remain in wells. First I have used 6% native PAGE. Then I used 4% stacking and 6%resolving, again DNA band...
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I have to identify an ancestral gene from a gene family, which has been duplicated many times and gave rise to a gene family. Please tell me any software which can help me.
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