Jibin Sadasivan

6 Questions 4 Answers 0 Followers

Questions related from Jibin Sadasivan

S2 cells fractionation

I am trying to prepare nuclear and cytoplasmic fractions from S2 cells. I want to separate nuclear and cytoplasmic RNA for Northern blot and sequencing. Can anyone provide protocols and...

06 July 2017 2,532 2 View

How can I separate/purify 2 KDa peptide after cleaving out GST tag ?

The 2 kDa GST Fusion protein was expressed as a in BL21DE3 expression system. After GST purification I want to remove the GST tag using precision protease and separate out the small peptide. I...

20 August 2015 7,028 4 View

Is it always that an alzheimers patients brain has both neurofibrillry tangles and beta amyloid plaques ?

Is there any relation between the activity of tangles and plaques ? Whenever tangles are formed that will definitely leads to the death of cells . How beta amyloid affects the activity of cell ?

25 October 2012 4,237 7 View

What is the importance of tissue glycoforms ?

Same proteins produced in different tissue have different glycosylation pattern.. How is this possible? What is the advantage of adding oligosaccharides to proteins ?

24 October 2012 8,648 2 View

How are the activities of beta amyloid plaques and neurofibrillary tangles realted ?

Neuro fibrillary tangles are formed by the aggregation of tau protein which ultimately results in the death of neuron, and beta amyloid is formed by the cleavage of beta secretase. Here what...

19 October 2012 6,964 3 View

What is the importance of beta secretase in non alzhimers patients?

Alpha-secretase helps in cleaving APP and hence eliminates the formation of beta-amyloid peptide and the left over APP that remains in the membrane is cleaved by gamma-secretase. What is the role...

14 October 2012 5,796 3 View