Recently, our core laboratory introduced this system, has anyone used this system to perform experiments? We are wondering whether it is worth it to spend money to buy the Impedance Sensing chip.
http://www.biophysics.com/ecis-theory.php
Hi Guan,
My researcg group has an about 4 year practice in impedimetry. We started with ECIS and actually we are working as a core facility in this field.
As you can also read from the literature ECIS has more models (ECIS 1600, ECIS Theta, ECIS Z Theta etc.) and the capacity of these models is ranging from the simple impedance measurement up to parallel registration of Z, R and C values in a real-time mode. The frequencies of AC applied are also varying up to 12 independent reading.
As the gold electrodes and the system is highly sensible there is a choice to use it to evaluate wound healing assays and electroporation, too.
The wide variety of arrayes provides the possibility to measure with single spot or multi spot electordes; in flow conditions and in 96 well plates ( and in several other choices).
Our practice showed that the minimum cell density is around 80-100.000 cell/well - but it depends on the cell type! In some conditions even some cells (!) were detected.
Up to now the following cell cultures were analysed with success in ECIS in our lab:
monocyte/macrophage - J774, THP-1, MM6
melanoma - A2058, WM35, HT168, HT-168-M1
endothels and fibroblasts as well as the ciliate Tetrahymena pyriformis.
(The last technique is described in a chapter "Tetrahymena pyriformis in motion" which you can find below: http://store.elsevier.com/Microbiorobotics/isbn-9781455778911/ pages 70-71)
See Wiertz et al impedance sensing for monitoring neuronal covarage and comparison with microscopy. IEEE Trans Biomed Engineering
http://www.nanoanalytics.de/en/hardwareproducts/cellzscope/index.php
i like it because it's not too expensive and there is no need for use special (...and expensive) consumables. You can use a lot of different systems so as Nunc, Greiner, Corning, Millipore etc...
regards
Jochen
We have been using impedance measurement equipment from Roche xCellIgence system in so many various ways starting from proliferation, cell signalling and ending with chemotaxis and invasiveness. Cells from primary cultures normal or tumoral, standard cell lines, separated peripheral NKs and so on were used.
I can elaborate on my available e-mail more.
Hi Monica,
Which system of xCELLigence do you use for chemotaxis measurement? I guess it was the DP as SP is dedicated for cell adhesion. Would you so kind and send me you publications oin that filed - we have both SP and DP, therefore all publications are very welcome on the filed. My address is [email protected]. Thanks in advance.
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