Solvent to dissolve std reduced GSH ?

The literature and data sheets mention the water as the best solvent to dissolve reduced GSH, they also mention the oxidization of GSH in an aqueous medium. But few protocols suggest using Buffer...

11 December 2019 7,024 2 View

What should be the Protein concentration for assays?

for CAT activity, is there is any threshold regarding the concentration of protein in the enzyme has to be taken into consideration before doing the experiment.

02 March 2019 7,719 6 View

Identification peak of Pb-nano particles ?

1. how to identify the characteristic peak of Pb-nano particles using UV-vis spectrometer, synthesized by green synthesis process ? 2. if its intra-cellular production is it possible to identify...

10 November 2018 7,938 3 View

What should be the concentration of methylene blue dye for distinguishing the live vs dead cells?

while working on the concept of distinguishing the live and dead cells by dye exclusion principle, widely TB, PI and Methylene blue (MB) are in use. In context to commercially available ready to...

05 June 2018 5,329 4 View

How to calculate Molarity ?

03 April 2017 4,648 6 View

Journal for publishing review article in mycology ?

06 July 2016 465 0 View

Different indicators in Citrate utilization test?

04 May 2016 9,148 2 View

Extraction of proteins from natural gums ?

03 April 2016 4,988 2 View

Colony PCR for filamentous fungi ?

Does it possible to do colony PCR for filamentous fungi using the spores for identification through Universal Primers ITS 1 and 4. thanks in advance

01 January 1970 694 24 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View