Effect of Sonication ?

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Steingrimur Stefansson

Hi Bhavya, sonication is usually included in purification steps to disrupt organelles and protein aggregates that cannot be solubilized easily by detergents alone.

Bhavya G.

Dear Stefansson,

thank you for your valuable answer.

Ahmed Dhamad

Yes, otherwise, you will lose a big amount of proteins specially insoluble ones. Good luck