Solvent to dissolve std reduced GSH ?

The literature and data sheets mention the water as the best solvent to dissolve reduced GSH, they also mention the oxidization of GSH in an aqueous medium. But few protocols suggest using Buffer...

11 December 2019 7,024 2 View

What should be the Protein concentration for assays?

for CAT activity, is there is any threshold regarding the concentration of protein in the enzyme has to be taken into consideration before doing the experiment.

02 March 2019 7,719 6 View

Identification peak of Pb-nano particles ?

1. how to identify the characteristic peak of Pb-nano particles using UV-vis spectrometer, synthesized by green synthesis process ? 2. if its intra-cellular production is it possible to identify...

10 November 2018 7,938 3 View

What should be the concentration of methylene blue dye for distinguishing the live vs dead cells?

while working on the concept of distinguishing the live and dead cells by dye exclusion principle, widely TB, PI and Methylene blue (MB) are in use. In context to commercially available ready to...

05 June 2018 5,329 4 View

Effect of Sonication ?

While doing the crude protein extraction from Yeast cells, for stress bio-marker assay, a. does the involvement of sonication step is crucial ? Thank you

03 April 2018 2,711 3 View

How to calculate Molarity ?

03 April 2017 4,648 6 View

Journal for publishing review article in mycology ?

06 July 2016 465 0 View

Different indicators in Citrate utilization test?

04 May 2016 9,148 2 View

Colony PCR for filamentous fungi ?

Does it possible to do colony PCR for filamentous fungi using the spores for identification through Universal Primers ITS 1 and 4. thanks in advance

01 January 1970 694 24 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View