Solvent to dissolve std reduced GSH ?

The literature and data sheets mention the water as the best solvent to dissolve reduced GSH, they also mention the oxidization of GSH in an aqueous medium. But few protocols suggest using Buffer...

11 December 2019 7,024 2 View

What should be the Protein concentration for assays?

for CAT activity, is there is any threshold regarding the concentration of protein in the enzyme has to be taken into consideration before doing the experiment.

02 March 2019 7,719 6 View

What should be the concentration of methylene blue dye for distinguishing the live vs dead cells?

while working on the concept of distinguishing the live and dead cells by dye exclusion principle, widely TB, PI and Methylene blue (MB) are in use. In context to commercially available ready to...

05 June 2018 5,329 4 View

Effect of Sonication ?

While doing the crude protein extraction from Yeast cells, for stress bio-marker assay, a. does the involvement of sonication step is crucial ? Thank you

03 April 2018 2,711 3 View

How to calculate Molarity ?

03 April 2017 4,648 6 View

Journal for publishing review article in mycology ?

06 July 2016 465 0 View

Different indicators in Citrate utilization test?

04 May 2016 9,148 2 View

Extraction of proteins from natural gums ?

03 April 2016 4,988 2 View

Colony PCR for filamentous fungi ?

Does it possible to do colony PCR for filamentous fungi using the spores for identification through Universal Primers ITS 1 and 4. thanks in advance

01 January 1970 694 24 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View