What should be the Protein concentration for assays?

for CAT activity, is there is any threshold regarding the concentration of protein in the enzyme has to be taken into consideration before doing the experiment.

02 March 2019 7,719 6 View

Identification peak of Pb-nano particles ?

1. how to identify the characteristic peak of Pb-nano particles using UV-vis spectrometer, synthesized by green synthesis process ? 2. if its intra-cellular production is it possible to identify...

10 November 2018 7,938 3 View

What should be the concentration of methylene blue dye for distinguishing the live vs dead cells?

while working on the concept of distinguishing the live and dead cells by dye exclusion principle, widely TB, PI and Methylene blue (MB) are in use. In context to commercially available ready to...

05 June 2018 5,329 4 View

Effect of Sonication ?

While doing the crude protein extraction from Yeast cells, for stress bio-marker assay, a. does the involvement of sonication step is crucial ? Thank you

03 April 2018 2,711 3 View

How to calculate Molarity ?

03 April 2017 4,648 6 View

Journal for publishing review article in mycology ?

06 July 2016 465 0 View

Different indicators in Citrate utilization test?

04 May 2016 9,148 2 View

Extraction of proteins from natural gums ?

03 April 2016 4,988 2 View

Colony PCR for filamentous fungi ?

Does it possible to do colony PCR for filamentous fungi using the spores for identification through Universal Primers ITS 1 and 4. thanks in advance

01 January 1970 694 24 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View