Easy method isolate synaptic mitochondria from mouse cortical tissue?

More Afzal Ali Misrani's questions See All

What is best loadning control antibody for mitochondira western blot?

As i have gone through many research papers, peoples used VDAC1/Porin or COX IV antibodies as mitochondria loading control. Is there any other loading control antibodies for mitochondria?...

03 April 2019 6,264 5 View

What is differnce between Potassium (K) EDTA and Sodium (Na) EDTA?

I want to isolate mitochondria from mouse brain tissue; For the experimental procedure of mitochondria isolation, a nature protocol used potassium EDTA to prepare EDTA solution and isolation...

03 April 2019 5,953 4 View

Is this logically fine to use sucrose preference test to evaluate depressive-like behavior in 24 h mouse model of total sleep deprivation?

Mainly sucrose preference test (SPT) protocol is 3 days training and 2 days test; as most reports follow, i am trying to find any SPT protocol which do not need training/habituation?

03 April 2018 5,512 5 View

How to detect low molecular weight molecules in western blot using 12% SDS Gel?

I am having problem with detecting low molecular weight in western blot, desired kda size is 15 and 10 kda. i use 12% SDS gel, soak the PVDF membrane in MeOH for 30 min before transfer, transfer...

01 January 1970 9,557 10 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Yang Huanhuan

Hoping this article will do.

Nashaat Ghalib

Hi there

You can try sucrose solution 0.25M in gradient centrifugation..It is so easy protocol.

Hoping you will do it successfully.

Saleh Alkarim

Hello

Kindly look at this article

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532608/

Good Luck

Afzal Ali Misrani

Thanks for sharing such a great articles. I will try it and back to you if have any problem.