Easy method isolate synaptic mitochondria from mouse cortical tissue?

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What is best loadning control antibody for mitochondira western blot?

As i have gone through many research papers, peoples used VDAC1/Porin or COX IV antibodies as mitochondria loading control. Is there any other loading control antibodies for mitochondria?...

03 April 2019 6,205 5 View

What is differnce between Potassium (K) EDTA and Sodium (Na) EDTA?

I want to isolate mitochondria from mouse brain tissue; For the experimental procedure of mitochondria isolation, a nature protocol used potassium EDTA to prepare EDTA solution and isolation...

03 April 2019 5,686 4 View

Is this logically fine to use sucrose preference test to evaluate depressive-like behavior in 24 h mouse model of total sleep deprivation?

Mainly sucrose preference test (SPT) protocol is 3 days training and 2 days test; as most reports follow, i am trying to find any SPT protocol which do not need training/habituation?

03 April 2018 5,457 5 View

How to detect low molecular weight molecules in western blot using 12% SDS Gel?

I am having problem with detecting low molecular weight in western blot, desired kda size is 15 and 10 kda. i use 12% SDS gel, soak the PVDF membrane in MeOH for 30 min before transfer, transfer...

01 January 1970 9,482 10 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Yang Huanhuan

Hoping this article will do.

Nashaat Ghalib

Hi there

You can try sucrose solution 0.25M in gradient centrifugation..It is so easy protocol.

Hoping you will do it successfully.

Saleh Alkarim

Hello

Kindly look at this article

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532608/

Good Luck

Afzal Ali Misrani

Thanks for sharing such a great articles. I will try it and back to you if have any problem.