What is best loadning control antibody for mitochondira western blot?

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Sajjeev Jagannathan

I have used Anti-VDAC1 / Porin from Abcam and find it good.

Kalpita R Karan

We use HSP60. Works well in cultured human fibroblasts.

Afzal Ali Misrani

@ Sajjeev Jagannathan thanks

Kalpita R Karan thanks

Waqar Ahmad

Be careful when using specific enzyme as antibody. Sometime they are directly involved in mitochondrial functions under study. How about structural proteins as antibodies.