What is differnce between Potassium (K) EDTA and Sodium (Na) EDTA?

I want to isolate mitochondria from mouse brain tissue; For the experimental procedure of mitochondria isolation, a nature protocol used potassium EDTA to prepare EDTA solution and isolation...

03 April 2019 5,686 4 View

Easy method isolate synaptic mitochondria from mouse cortical tissue?

Recently i am trying to isolate synaptic mitochodia from accure brain tissue; as i am new to this technique, can anyone please share with me easy to follow protocol? Thanks in advance.

02 March 2019 3,352 4 View

Is this logically fine to use sucrose preference test to evaluate depressive-like behavior in 24 h mouse model of total sleep deprivation?

Mainly sucrose preference test (SPT) protocol is 3 days training and 2 days test; as most reports follow, i am trying to find any SPT protocol which do not need training/habituation?

03 April 2018 5,457 5 View

How to detect low molecular weight molecules in western blot using 12% SDS Gel?

I am having problem with detecting low molecular weight in western blot, desired kda size is 15 and 10 kda. i use 12% SDS gel, soak the PVDF membrane in MeOH for 30 min before transfer, transfer...

01 January 1970 9,482 10 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View