Afzal Ali Misrani

5 Questions 11 Answers 0 Followers

Questions related from Afzal Ali Misrani

What is differnce between Potassium (K) EDTA and Sodium (Na) EDTA?

I want to isolate mitochondria from mouse brain tissue; For the experimental procedure of mitochondria isolation, a nature protocol used potassium EDTA to prepare EDTA solution and isolation...

04 April 2019 5,881 4 View

What is best loadning control antibody for mitochondira western blot?

As i have gone through many research papers, peoples used VDAC1/Porin or COX IV antibodies as mitochondria loading control. Is there any other loading control antibodies for mitochondria?...

04 April 2019 6,236 5 View

Easy method isolate synaptic mitochondria from mouse cortical tissue?

Recently i am trying to isolate synaptic mitochodia from accure brain tissue; as i am new to this technique, can anyone please share with me easy to follow protocol? Thanks in advance.

03 March 2019 3,383 4 View

Is this logically fine to use sucrose preference test to evaluate depressive-like behavior in 24 h mouse model of total sleep deprivation?

Mainly sucrose preference test (SPT) protocol is 3 days training and 2 days test; as most reports follow, i am trying to find any SPT protocol which do not need training/habituation?

04 April 2018 5,492 5 View

How to detect low molecular weight molecules in western blot using 12% SDS Gel?

I am having problem with detecting low molecular weight in western blot, desired kda size is 15 and 10 kda. i use 12% SDS gel, soak the PVDF membrane in MeOH for 30 min before transfer, transfer...

01 January 1970 9,525 10 View