Why science doesn't work without curiosity?

Curiosity: It Helps Us Learn, But Why?

05 June 2017 8,720 99 View

What Is Greenhouse Gas?

Read 12 answers by scientists with 33 recommendations from their colleagues to the question asked by Ahmed Madi Al Mayahi on May 14, 2017

04 May 2017 2,888 12 View

Red blood cells their color red, of course , What is the color of White blood cells?

Read 1 answer by scientists with 1 recommendation from their colleagues to the question asked by Ahmed Madi Al Mayahi on Mar 18, 2017

02 March 2017 8,933 1 View

How do migrating organisms find their way?

Birds, butterflies, and whales make annual journeys of thousands of kilometers. They rely on any thing to find their way?

31 December 2016 5,286 11 View

How do you see your experience in registration in site of research gate?

Scientific research, questions, answers etc...

11 December 2016 6,909 6 View

What is the basis of variation in stress tolerance in plants?

Read 13 answers by scientists with 54 recommendations from their colleagues to the question asked by Ahmed Madi Al Mayahi on Dec 27, 2016

11 December 2016 8,635 13 View

Is Breakfast Really the Most Important Meal of the Day?

The idea that breakfast is the most important meal of the day has been ingrained in our minds. But is it true?

11 December 2016 645 11 View

Any of the plant parts are firstly affected by salinity ?

Cultured plants are exposed to many stresses, and salinity of the most important stresses that affect on plants in many regions of the world.

11 December 2016 8,382 5 View

Show in some times poor growth of plants cultured in glass vessels due to vitrification? What are the reasons that lead to the emergence of that case?

10 November 2016 248 2 View

What is the effect of pH adjusting (5.7-5.8) for media on the plant cell ?

absorption , growth , semi solid or liquid media etc...

10 November 2016 2,365 0 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View