How to calculate the band potential of a semiconductor material using energy band gap?

I am looking for methods to calculate the band potential of ZnFe2O4 and Fe2O3.

05 January 2021 6,416 2 View

I am confused about the number of active sites in enzymes. Is there any enzyme with multiple active sites?

I want to know about the number of active sites in an enzyme.

15 December 2020 231 9 View

Besides, DMSO, EDTA, and K2Cr2O7 to scavenge the OH radicals, holes, and electrons respectively what are the other chemicals being used for scavengin?

Besides, DMSO, EDTA, and K2Cr2O7 to scavenge the OH radicals, holes, and electrons respectively what are the other chemicals being used for scavenging? Please share the files with complete...

30 November 2020 4,935 1 View

Which procedure is most convenient for performing tensile strength tests on reinforced laboratory soil specimens?

The fact that there are no proper laboratory equipments devised till date for direct tensile testing of soil, I'm looking for a simple indirect procedure that would help in determining tensile...

12 October 2020 8,231 2 View

How Geochemical models help in solution chemistry in the experiment?

Added Si to nutrient solution carrying metals and mineral nutrients under acidic conditions, therefore, it's necessary that they provide evidence that Si addition does not cause pH fluctuations...

26 September 2020 7,706 2 View

Which technique is better to use in Hierarchical Linear Regression, SEM or Process Macro and Why?

My model consisted of one independent variable, two moderating variables, and one dependent variable. Which technique is better to use in Hierarchical Linear Regression, SEM or Process Macro and...

13 November 2019 240 3 View

How will Hedgehog gene expression change by using Hedgehog inhibitors like cyclopamine?

Should it increase or decrease?

04 January 2019 8,268 1 View

Can I work on the foreign aids impact on sectoral GDP like Agricultural Sector , Industrial Sector as a panel data analysis ?

I want to investigate the impact of foreign aids on sectoral growth including three sectors but as a panel data analysis ,I found a lot of time series studies that include more than one sector ,...

17 September 2018 2,803 7 View

What are the possible reasons for plasmid extraction failure?

I'm trying to extract my constructed plasmid from E.Coli Top 10 cells for which colony PCR is positive and failed multiple times. Plasmid is pET-28a. I'm using Tiangen Mini Plasmid kit and the...

07 August 2018 9,798 7 View

What is the different between LNCaP and LNCap-LN-3 Cell Line? Is LNCaP-LN3 cell line can be use to develop prostate cancer model in nude mice?

In the literature, LNCap is lymph node metastasis cell line obtained from Caucasian male and LNCaP-LN-3 is metastatic subline of LNCaP cells derived by orthotopic implantation. I want to know...

04 July 2018 2,779 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View