Regarding the research design, which is the best way to investigate childrens' emotional trauma and the effects on their learning?

Hi all, I am in the process of thinking about the research design of my masters' dissertation and my topic is the emotional trauma of primary school children and the effects on their learning....

14 March 2018 8,304 3 View

Yersinia enterocolitica ATCC 9610 biovar 1A or 1B?

Yersinia enterocolitica ATCC 9610 is described as being biovar 1, serogroup O:8. Is there anyone that has tested the strain to clarify whether it is 1A or 1B biotype?

17 March 2016 3,458 1 View

Any advice on Thermal Release Tape suppliers in Europe?

Hello, I am interested in getting some thermal release tape suitable for graphene transfer. Anyone knows suppliers in Europe so that I can avoid the sipping cost? Thank you

03 November 2015 4,766 3 View

Personal Learning Enviroment and online teaching

How does a PLE assist teachers and students in online teaching and what potential does it have?

01 January 1970 5,902 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View