After completion of CTAB method of DNA extraction and completing the final step of ethanol wash the DNA is left for drying. Since air drying takes a longer time, it is suggested to keep the Microfuge tube containing DNA pellet in a hybridization oven at 37-degree celsius. Will keeping the DNA in hybridization oven for drying make it hard and difficult to liquify further? or is air drying the better method
Sesha is right. If you dry too much it will be difficult to dissolve DNA in any solvent of your choice. From my experience, air drying is the best. After decanting the last bit of ethanol out of the tube, I usually have the tubes up side down and tilted against the rack for air flow. This prevents the residual ethanol dripping back onto DNA. Instead the ethanol remains on the wall of the tube and drys off quicker.
Try it! Good luck!
Dont dry it too much as it wont dissolve later. Vaccum evaporation may be done and dissolved in sterile water or TAE
Sesha is right. If you dry too much it will be difficult to dissolve DNA in any solvent of your choice. From my experience, air drying is the best. After decanting the last bit of ethanol out of the tube, I usually have the tubes up side down and tilted against the rack for air flow. This prevents the residual ethanol dripping back onto DNA. Instead the ethanol remains on the wall of the tube and drys off quicker.
Try it! Good luck!
It really depends on what you plan to do with the DNA. If there is any ethanol remaining, it will affect sequencing. If you plan to dissolve your DNA a high dilution (and remember that if you dissolve in water, it will be singlestranded) then traces of ethanol might not be important. I usually suck air over the pellet using a fine plastic tube attached to a water aspirator - you can watch the ethanol disappear in a few seconds. Then dissolve in 10mM Tris-HCl pH 7.2 + 1mM EDTA. Note that pure dry DNA will dissolve with perseverance. If there is still protein contamination, it takes a lot longer, but it is not insoluble.
Aspire as much of the remaining 70% ethanol as you dare (provided you know where your pellet is), with a glass capillary. Then dry you pellets for 5 minuts in a vacuum dessicator. Dissolve the DNA in a buffered solution (TRIS-HCl pH 7.5) Using this strategy, it might be helpfull to use NH4Acetate as precipetation salt, since this evaporates as acetic acid and ammonia during the vacuum drying step
yes, Eun D.Lee is right
Air drying is the best condition for drying your DNA.
Mostly if you need to resuspend in 10mM Tris-HCl pH 7.2 + 1mM EDTA buffer.
good luck!
be patient+++
Try to remove as much ethanol as possible by keeping the tubes in inverted position and then you can air dry it. Air drying sometimes takes longer so I prefer to vaccum dry at 30 degrees for five to ten minutes.
During my research I dried DNA in air at room temperature for 5 minutes. For PCR is was enough.
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