Sheetal Bandhu

4 Questions 13 Answers 0 Followers

Questions related from Sheetal Bandhu

Can anyone tell me how to prepare a standard callibration curve for isoprene which is a volatile compound ?

Isoprene is a 5 carbon hydrocarbon released by some bacterial species. My question is how to do sampling of isoprene which a volatile compound. Second point is how to prepare a standard...

08 August 2018 2,790 0 View

How can I concentrate total RNA samples?

I have isolated total RNA from a yeast by using a kit, but the concentration is not very high. How can I concentrate my existing RNA samples?

05 May 2016 6,514 4 View

Is it possible for an enzyme to have more than one pH optimum, and why ?

A partially purified protein showed three pH optimas. For the enzyme assay same substrate and coenzyme were used at all pH values. What can be the reason for it ?

10 October 2015 9,560 2 View

Can anyone assist with PCR amplification of my gene?

I am not getting amplification of my gene. Its gc content is 53 % do I need to use supplementary substances like DMSO etc, its Tm is ~62 degree. the primers have been designed with restriction...

08 August 2014 5,238 13 View