Doubled haploid barley production?

29 October 2013 0 9K Report

Does anyone work with doubled haploid barley production? I’m looking for optimum media or shock pretreatment of ears/anthers. I have used cold pre-treatment for 14 days at 4°C before, 0.3M liquid mannitol and 0.7M solid mannitol. Any suggestions?

Badges
Science topic

More Ewa Nowak's questions See All

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

I am considering injecting cisplatin directly into an equine sarcoid tumour that is chronically refractive?

My case has been under treatment now for 3 years with little response. Now considering euthanasia. Last ditch is Cisplatin for dilution in iv infusion (100ml/ml in saline and mannitol). What can I...

05 August 2024 1,835 0 View

My question is about a barley seed ?

I need some articles on the analysis of the nutritional quality of barley (H.vulgare.L) and the analytical approach of metabolomics and proteomics.

26 June 2024 3,451 2 View

How can I purify filamentous fungi from leaf tissue?

I want to perform PacBio RNA-seq of a filamentous fungal isolate during multiple infection time-points in barley leaf. I was told that PacBio RNA-seq would not be viable due to the low...

12 June 2024 4,027 1 View

What does mannitol do in isolation buffer...?

I am preparing isolation buffer for mitochondrial isolation purpose. In protocol, it is given to add 225 mM sucrose and 75 mM mannitol for preparing isolation buffer. I do not have mannitol in my...

01 June 2024 5,936 1 View

Can Achromobacter grow on nitrogen free mannitol broth?

We isolated azotobacter from soil for cellulose production but 16srrna identified it's Achromobacter.

13 March 2024 9,352 0 View

Request of information about Indigestible marker Titanium Dioxide "TiO2"?

To evaluate the nutritional value of hulless barley in poultry nutrition I performed an experimental trial last summer, and I used TiO2 as a marker (at a rate of 5 g/kg) to evaluate the amino acid...

13 February 2024 9,262 8 View

To test chemical composition of different biomass types?

I want to test the chemical composition of Barley straw and Brown Algae biomass. By chemical composition, I mean testing Cellulose, Hemicellulose, and Lignin content in Barley Straw, while testing...

28 December 2023 8,873 4 View

Resources for specific dietary fiber composition of feed ingrdients?

Hi, I am looking for a resource that can show me the dietary fiber composition of certain feed ingredients in animal feed. For example I want to know the main types of hemicellulose fibers...

07 December 2023 6,351 0 View

Which medium is the best to be used for qualitative estimation of IAA production of PGPR?

As per the tiltle said, I want to qualitatively test how much IAA can be produced by some of the bacteria that I have just isolated from a soil sample. After culturing the soil bacteria in a...

03 December 2023 4,408 1 View

Which methode we should use to find Variation of Trace Element Content in Highland Barley Grain. Fe, Zn, Mn, Se, Cu???

which method we have to use to determine the concentration of Micronutrient (Zn, Fe, Mn,Se, and Cu)

23 October 2023 6,930 0 View