Did you check the original marker substance? Did it fulfill the claimed composition?

Yes, I bought the marker from a lab. according to the datasheet, however, I didn't check it in the laboratory, but I'll try to perform the calibration curve using the technique of Short et al., 1995 or the technique of Ashley et al., 2022.

Thanks Professor.

Conduct an acid digestion process on the TiO2. Create a plot using your standards, and this will confirm whether it is adulterated or not. This step will help eliminate one unknown from the analysis.

Great thanks Emmanuel, I'll perform the process till I receive the H2O2 (30%), which will be in 2 or three days.

Thanks a lot

Titanium dioxide (TiO2) is commonly used as an indigestible marker in animal nutrition studies to estimate nutrient digestibility, particularly in feed trials involving poultry, pigs, and ruminants. Here's some information about TiO2 as an indigestible marker:

In summary, Titanium dioxide is a widely used indigestible marker in animal nutrition research to estimate nutrient digestibility and assess feed efficiency. Proper experimental design, sample collection, and analytical techniques are essential for accurate and reliable results when using TiO2 as a marker in digestion studies.

Thanks a lot sir Amenu. That's right, but we don't have any government agency conerning th topic, for this reason, we find reat problems to conduct experiemtnal trials in Animal nutrition. Unfortunately.... This is Scientific Research in Algeria.

Hell Dear, may be here are the solutions,

The issue you're encountering, where no titanium dioxide (TiO2) content was detected in both the feed and ileal digesta, could be due to several potential reasons. Here are some possible explanations and troubleshooting steps to consider:

1. Sampling and Mixing Issues

• Uniform Mixing: TiO2 might not have been uniformly mixed into the feed. If the marker was not evenly distributed, some samples might not contain any TiO2.Solution: Ensure thorough and homogeneous mixing of TiO2 in the feed. Consider using a mixer designed for fine particle distribution and perform multiple mixing steps if necessary.

2. Analytical Method Issues

• Method Sensitivity: The analytical method used might not have been sensitive enough to detect TiO2 at the levels present.Solution: Confirm that the analytical method (Short's method, 1996) is validated for the TiO2 concentration used (5 g/kg) and is appropriate for your sample matrix.

3. Sample Contamination or Loss

• **Contamination or Loss During Preparation

4o

To evaluate the nutritional value of hulless barley in poultry nutrition I performed an experimental trial last summer, and I used TiO2 as a marker (at a rate of 5 g/kg) to evaluate the amino acid digestibility. The samples of diets and ileal digesta have been sent to a laboratory for analysis. However, after the samples' preparation, the results showed no TiO2 content in the feed and ileal digesta (using Short's method, 1996). Till now, I don't understand the situation, and where is the problem.

ChatGPT

It's concerning that you're not finding any TiO2 content in the feed and ileal digesta despite using it as a marker. Here are some potential issues and solutions to consider:

By investigating these potential issues and implementing appropriate solutions, you can hopefully identify the source of the problem and ensure accurate evaluation of the nutritional value of hulless barley in poultry nutrition using TiO2 as a marker.

thanks, sir, the problem has been detected, the TiO2 used has been not soluble. However, I found another source I for which I did the analysis, it has give a positive result.