Can nonanol be directly oxidized into nonanal by O2 under ambient conditoions without any catalysts or additives?

Can nonanol be directly oxidized into nonanal by O2 under ambient conditoions without any catalysts or additives? any comments and literature would be appreciated.

09 November 2020 6,744 5 View

How to use pilot data to do simulation to calculate the sample size for linear mixed model using matlab?

Hi, all, I have a 2(group)*3(time) design, and at this moment, I got 5 subjects in each group in the pilot study. I would like do a power analysis and sample size calculation. since the design I...

03 February 2020 8,851 1 View

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If the mass and volume of the oil droplet are known, and the oil droplet is formed from nonanoic acid, how to calculate the cross-sectional area and thickness of the oil droplet on the water...

28 December 2019 705 3 View

How to present the relationship between two mutually exclusive independent variables on dependent variable in one model?

I want to compare the different impact of two communication strategies (stra. A and stra. B) on brand trust. Meanwhile, the main effect between communication strategy and brand trust is mediated...

20 June 2019 5,122 3 View

Why the distance between bromophenol blue bands and xylene cyanol FF bands are close?

I am doing the gel electrophoresis to check my DNA samples, and I found that after adding loading buffer and start running, the distance between bromophenol blue bands and xylene cyanol FF bands...

04 June 2019 2,300 3 View

What are the bandgap scope of carbon quantum dots with blue,cyan and green fluorescence, respectively?

What are the bandgap scope of carbon quantum dots with blue,cyan and green fluorescence respectively? such as red fluoresence carbon quanutm dots correspond to ca.2.06eV. How about the others?

18 December 2017 9,681 2 View

Why i cannot get a good curve of acarbose in alpha-glucosidase assay?

I had tested Acarbose in Alpha-glucosidase assay, the result showed a very low absorbance, it almost same reading as negative control (Distilled water). Below is my method of the assay. Anyone...

08 December 2017 2,457 2 View

Can ATP be separated from AMP an ADP by silica gel TLC plate? If so, what is the best developing system?

How to purify ATP in a large quantity, like 1 gram? Is it possible to run a silica gel column instead of a ion-exchange column? Thanks.

09 November 2017 5,943 5 View

My research show fungi grew in PDA contained high conc. heavy metal but did not grew in low conc. heavy metal, why this happened?

my research show fungi grew in PDA contained high concentration heavy metal but did not grow in low concentraion heavy metal, why this happened? Because in theory, the fungi did not grow in high...

02 May 2017 994 3 View

For Chip-seq, how to design primers for qPCR validation of purified DNA after imunoprecipation?

26 March 2017 1,435 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View