Dot Blot protocol - FAQS.TIPS

More Dai Quach's questions See All

Is there any materials that can be as flexible and stretchable as PDMS, while also having a small linear thermal expansion coefficient?

For the PDMS material, it's linear thermal expansion coefficient is about 3.2*10-4/℃. The polyimide has a smaller linear thermal expansion coefficient like 2*10-5/℃, but it cannot be stretch. It...

04 July 2024 4,525 1 View

How to eliminate imaginary phonon frequency in phonon dispersion of Coved Graphene Nanoribbons?

Dear all, I want to calculate the phonon dispersion of 4CNR-1-0 (structure is in Fig. 2 (b) in this article Designing coved graphene nanoribbons with charge carrier mob... ) and then calculate...

17 June 2024 6,911 2 View

How long can lyophilized cycloheximide be stored at 4℃?

I found a bottle of lyophilized cycloheximide in the lab refrigerator (4℃). I think lyophilized form should be rather stable, but it's been open for 3.5 years. Cell signaling says the lyophilized...

11 June 2024 8,366 0 View

How to measure the bisulfite concentration?

Does anyone know the exact BS concentration of the Zymo DNA methylation gold kit? or is there anyway to measure the BS concentration by titration? Thanks.

05 May 2024 8,848 1 View

What are the 3’ end modifications that prevent DNA from being extended by DNA polymerase? Which one has the best blocking effect? Leakage is minimal?

01 May 2024 5,188 5 View

How do we do prestressed concrete beam in LS Dyna with proper initial stress and strain in the precast portion of the concrete beam?

I am facing a problem with the simulation of the precast concrete beam with prestressing. Typically, a precast beam would be fabricated in the factory with prestressing tendons (precambering) to...

06 February 2024 1,046 1 View

Can BHQ quench the fluorescence of FAM in the case shown below?

28 January 2024 6,182 1 View

Migration in Lasius ants?

Do ants in the genus Lasius migrate? If so, what are some of the characteristics of their migratory routes? Cheers

03 December 2023 6,320 3 View

How dose CO2 transport the membrane of methanogenic archaea?

Hi, everyone. May I ask if anyone knows the specific mechanism by which carbon dioxide (CO2) passes through the cell membrane of methanogenic archaea, is it similar to that of bacteria?

13 September 2023 9,548 0 View

How to make this medium?

Hi, I want to make a medium called BYGT. According to the paper, it consists of 1.9% Brain heart infusion, 0.5% Yeast Extract, 0.2% Glucose, and 0.1 volume of 1.0M Tris at pH8. If I want to make...

09 August 2023 5,838 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM images?

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?

25 July 2024 5,482 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Issues with Malaria Coated Nitrocellulose Membrane Releasing Moisture?

Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...

16 July 2024 7,937 1 View

How to detect phosphorylation on Western Blots?

Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?

15 July 2024 2,055 3 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Is there anyway to distinguish two proteins of same molecular weight?

I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...

14 July 2024 9,324 2 View