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I am looking for any papers that mentioned about using puromycin in cell selection after transfection. I already got the optimized of MTX and G418. Then I started cell selection with puromycin at...
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After running Elisa, i ran gel and western to confirm the results. I have a question with the densitometer. I have the problem to quantitate the concentration of the protein based on the bands in...
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Why do we have to wash the membrane again with TBS only after wash the 3 times with TBS-T ? I I don't wash it again, what happen ?
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We have developed a very successful process of running Sp2 cells in 250mL shake flask, 2L bioreactor, 5L bioreactor, in 10L bioreactor for the last two years. The cultures lasted 12 - 14 days....
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