Hi, everyone. May I ask if anyone knows the specific mechanism by which carbon dioxide (CO2) passes through the cell membrane of methanogenic archaea, is it similar to that of bacteria?
For the PDMS material, it's linear thermal expansion coefficient is about 3.2*10-4/℃. The polyimide has a smaller linear thermal expansion coefficient like 2*10-5/℃, but it cannot be stretch. It...
04 July 2024 4,525 1 View
Dear all, I want to calculate the phonon dispersion of 4CNR-1-0 (structure is in Fig. 2 (b) in this article Designing coved graphene nanoribbons with charge carrier mob... ) and then calculate...
17 June 2024 6,911 2 View
I found a bottle of lyophilized cycloheximide in the lab refrigerator (4℃). I think lyophilized form should be rather stable, but it's been open for 3.5 years. Cell signaling says the lyophilized...
11 June 2024 8,366 0 View
Does anyone know the exact BS concentration of the Zymo DNA methylation gold kit? or is there anyway to measure the BS concentration by titration? Thanks.
05 May 2024 8,848 1 View
What are the 3’ end modifications that prevent DNA from being extended by DNA polymerase? Which one has the best blocking effect? Leakage is minimal?
01 May 2024 5,188 5 View
I am facing a problem with the simulation of the precast concrete beam with prestressing. Typically, a precast beam would be fabricated in the factory with prestressing tendons (precambering) to...
06 February 2024 1,046 1 View
Can BHQ quench the fluorescence of FAM in the case shown below?
28 January 2024 6,182 1 View
Do ants in the genus Lasius migrate? If so, what are some of the characteristics of their migratory routes? Cheers
03 December 2023 6,320 3 View
In SmartPLS, I used a reflective-reflective model. In the first order model assessment, the reliability is fine. And the validity is fine. My validity assessment included cross loadings,...
06 August 2023 1,636 0 View
Extraction Method: cold enzyme assay buffer [4℃,10 mM nicotinamide, 20 mM sodium bicarbonate, 100 mMsodium carbonate (Na2CO3), pH 11; a buffer classically usedfor enzymatic NAD(P)(H) analysis];...
23 July 2023 3,458 3 View
So, I am working on a small protein (X) of Haloferax volcanii. It shows these results: 1. Mutant strain grows poorlycompared to wild type strain under low iron condition. 2. Growth of Mutant...
14 May 2024 9,037 4 View
I'm trying to get a better yield for DNA/RNA extraction on Sulfolobus (Archaea), And I have tried SDS and it did not gives a good yield of DNA/RNA. here is a little information about Sulfolobus:...
02 May 2024 1,032 1 View
I want to culture methanogens from the fecal sludge sample. Which culture media should I use?
14 March 2024 571 0 View
What are the current recognized archaeal phyla/kingdoms? I'm in need of their names, and possible resources to back them up. I only need the names of the main groups within the archaea domain, but...
19 February 2024 6,508 1 View
Metabolically engineered strains in the sense of overexpression or deletion of specific genes within their metabolic pathways, enabling the transfer of biosynthesis pathways between different...
19 December 2023 6,822 1 View
Anaerobic digestion of rice straw and food waste designed for hydrogen production ended up producing more nitrogen, and less hydrogen. The inoculum is a seed sludge, (the source/details are...
01 December 2023 3,394 4 View
I hope everyone is doing well! I was wondering if someone could please help me in that I want to discover Lineage-Specific proteins in a genus of archaea using Orthofinder. Therefore, could...
05 October 2023 5,498 1 View
PeiR is a lytic enzyme which is a methanogen virus that infects Methanobrevibacter ruminantium M1. Do all protease have active sites, if so how do I find it with the sequence only.
12 July 2023 8,548 4 View
I am doing ex-situ biomethanation and as I intensify my process I have encountered foaming. Have you had similar experience and if yes do you know of any antifoaming agents that does not affect...
18 June 2023 8,365 1 View
Dear all, I am currently trying to determine the number of archaea present in an ecosystem with the 16S rRNA gene. I am normalizing the mcrA gene with the total archaea 16S rRNA gene. The mcrA...
16 March 2023 1,609 4 View