I am working on primary neuronal culture. I have two drugs, suppose A and B. how can we determine various concentrations of those drugs have to be made to give a dose to the cells?
Please provide the context of the cells, drugs and the experiment. Is the effect to be measured a toxicity screen? If proliferation rate of the cells need to be determined in varying concentrations of these drugs, then a simple kill curve experiment can determine the dosage required to kill half the cells in a given time. Most drugs and their effects are known with the required concentrations, so please do a literature survey to find what you are looking for, thanks.
Prepare a small gradient of concentrations (1nM, 10nM, 100nM, 1uM, 10uM, 100uM, 1mM, 10mM) from lower nanomolar to millimolar range. This will give you an idea about what range the IC50 would fall around. Now you can set up more concentrations around that particular range to estimate the IC50 of the drug with more accuracy.
Aadil Javed thank you, sir. Those drugs are routinely used in the treatment of neuropathic pain. also, the drugs are working as adjuvant, supplements. so, no need for toxicity screening. drugs will be used to make a particular dose to the primary neuronal culture. as you suggested, I did a literature survey in which I found that some of the literature was used the concentration 500mM. I want to understand that, how we could find that figure which we will be used in our experiment?
Praveesh Valissery thanks sir, I have one more question. if we do prepare the concentrations with the help of the bioavailability of the drugs, should we go for the IC50? please guide me more about the determination of IC50 or can you suggest to me any readings?
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