I was trying to culture glial cell from embryonic day 17th. But, cells were not survived. I provided the DMEM+10% FBS and glutamax. Even though the cell are not differentiating into glial cells. whenever I use same cell to grow neurons, cells are growing as neuron in neurobasal medium. So, what am I supposed to do? please suggest me steps that I could improve my protocol.

Thanks in advance.