Hi here, I bought this product "https://avantilipids.com/product/840875" in powder form. My protocol for lipid nanoparticle synthesis uses a microfluidic system from PreciGenome. I need to dissolve the lipid in ethanol for nanoparticle synthesis. I tried ethanol and ethanol-chloroform-methanol (major-minor-minor portion); but it is not completely soluble. I would really appreciate it if you could please suggest a method to dissolve DOPA.
Dhiraj Kumar The product description for 18:1 PA (1,2-dioleoyl-sn-glycero-3-phosphate (sodium salt) on the Avanti Polar Lipids website states that it is soluble in chloroform. It is possible that the lipid is not completely soluble in ethanol due to its chemical properties. Ethanol is a polar solvent, and some lipids may not dissolve well in polar solvents. You may want to try using a non-polar solvent such as hexane or ether instead of ethanol to dissolve the lipid.
Hi everyone, thank you for the suggestions. I highly appreciate the response. I managed to dissolve the PA in 70-75 ethanol (with water).
DOPC in 100% ethanol (5 mg/ml).
DOPA/PA in 70% ethanol (5 mg/ml).
PE in 85% ethanol (5 mg/ml).
PE-PEG 2000 MW in 95% ethanol (5-8 mg/ml).
Cholesterol in 100% ethanol (5-8 mg/ml).
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