I am trying to optimize the dose of drug for neuronal cell culture. How do I determine EC50 value? Suggest literature and easiest method and calculations. is checking the cell viability acceptable to determine EC50 values or have to consider other parameters also? . How can dose response curve will be used to calculate EC50? Please guide
Hello Dhiraj Shivsharan
How do I determine EC50 value?
Use the drug dose-response curve to determine EC50 value. This curve is used to test drug efficacy invitro using cell lines.
Is checking the cell viability acceptable to determine EC50 values or have to consider other parameters also?
Checking cell viability is enough to determine the EC50 value.
How can dose response curve will be used to calculate EC50?
The EC50 is the concentration of the drug that provokes a response halfway between the baseline (Bottom) and maximum response (Top). It is impossible to define the EC50 until you first define the baseline and maximum response. You may refer to the link below to calculate the EC50 from dose response curve.
https://towardsdatascience.com/drug-dose-response-data-analysis-5d7d336ad8e9
Suggest literature and easiest method and calculations.
You may also refer to the link below.
https://www.aatbio.com/tools/ec50-calculator
Best.
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